https://www.cytomad.com/blog daily 1.0 2018-02-07 https://www.cytomad.com/blog/2017/10/3/9-tricks-you-can-use-to-improve-your-sort monthly 0.5 2017-10-09 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59daf38ccd0f688947e4c946/1507523225807/viability.png BLOG - 9 Tricks You Can Use to Improve Your Sort In this example the post-sort purity check showed a GFP+ fraction that appeared to be lower than the pre-sort expression. By adding a viability dye we were able to determine that the GFP- fraction were cells that had died during the sort process, and in fact the post-sort purity of viable cells was 99.4%. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59dade1e4c0dbf0142bd701e/1507523225805/Blausen_0909_WhiteBloodCells+%281%29.png BLOG - 9 Tricks You Can Use to Improve Your Sort Image from Blausen.com staff (2014). "Medical gallery of Blausen Medical 2014". WikiJournal of Medicine 1 (2). DOI:10.15347/wjm/2014.010. ISSN 2002-4436. (Own work) [CC BY 3.0], via Wikimedia Commons https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59db5fd0a8b2b06fbe17aac9/1507549156044/Sorting+buffer+recipe.png BLOG - 9 Tricks You Can Use to Improve Your Sort Adapted from Buffer Suggestions for Cell Sorting from FCRC at Rockefeller University https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59dad776edaed8465319b57f/1507523225803/IMG_6431.JPG BLOG - 9 Tricks You Can Use to Improve Your Sort https://www.cytomad.com/blog/2017/10/8/the-virtue-of-viability monthly 0.5 2018-02-20 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a69682c24a6946fda0e7cf1/1516857420881/spillover.png BLOG - The Virtue of Viability Propidium Iodide is a very useful dye, particularly in less fluormetrically complex assays, but in multicolour assays the broad emission profile of the dye can result in significant compensation problems across multiple channels. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a696e390d929731fd178792/1517285671973/all+cells.png BLOG - The Virtue of Viability Events in this data set were only gated on classical FSC vs SSC parameters, and 2 distinct lymphocyte-like populations are seen. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a696123ec212d357d7d4b04/1516855635369/membrane+permeability.png BLOG - The Virtue of Viability When using membrane impermeant DNA binding dyes, cells with an intact membrane will exclude the dye and remain negative for fluorescence. Cells with a compromised membrane will take up dye and show positive fluorescence. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a696dfc41920243675edb52/1516858879623/spectral+overlap+live-dead.png BLOG - The Virtue of Viability In this example, the Propidium Iodide has been replaced with Live/Dead Fixable Red Stain, which has a notably narrower emission profile, resulting in less spectral spread and improved resolution of data in multicolour panels. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a696e480d929731fd17898c/1517285576469/viable+cells+only.png BLOG - The Virtue of Viability When a viability dye is added (in this case TO-PRO-3), it becomes apparent that one of these populations is made up of membrane compromised cells as the left population disappears when excluding cells based on dye uptake. Furthermore, downstream gating shows that when the dead cells are excluded, the percentage of CD3+ cells drops markedly. The viability dye has eliminated a significant proportion of false positive events. https://www.cytomad.com/blog/2017/10/17/time-saving-your-data-for-free monthly 0.5 2018-01-25 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a6942c79140b716ad10cd42/1516847821911/all+fluors.png BLOG - Time: Saving Your Data for Free Figure 3. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a693ce5f9619adf7948f2ef/1516846317344/Time+gated.png BLOG - Time: Saving Your Data for Free Figure 2 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a693cce8165f5523ad78687/1516846293833/Ungated.png BLOG - Time: Saving Your Data for Free Figure 1. https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a69566fc83025d0fbf9d0d0/1516852856042/don%27t+waste+time+%281%29.png BLOG - Time: Saving Your Data for Free https://www.cytomad.com/blog/category/data monthly 0.5 https://www.cytomad.com/blog/category/gating monthly 0.5 https://www.cytomad.com/blog/tag/multicolour monthly 0.5 https://www.cytomad.com/blog/tag/data monthly 0.5 https://www.cytomad.com/blog/tag/reproducibility monthly 0.5 https://www.cytomad.com/blog/tag/sorting monthly 0.5 https://www.cytomad.com/blog/tag/gating monthly 0.5 https://www.cytomad.com/blog/tag/time monthly 0.5 https://www.cytomad.com/blog/tag/sample+preparation monthly 0.5 https://www.cytomad.com/blog/tag/viability monthly 0.5 https://www.cytomad.com/images daily 0.75 2018-01-25 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5a69338b0d929731fd0b2b8c/1507200672388/ Images - Brand New Astrios https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/59d37a8b51a584ef26baa562/1507200672388/Beckat.jpg Images - Brand New Astrios https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/59d60f11a9db097762a6d6db/1507200925220/unnamed.jpg Images - Astrios - first biological sample https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/59d60ebaa803bba2527f35a4/1507200832996/unnamed-4.jpg Images - Dark denim must be protected from bleach at all costs! https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/59d60e4349fc2b1e317daa25/1507200775386/unnamed-2.jpg Images - Non-PC2 Labcoat https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/59d6fb34f9a61e180f584c81/1507261294094/FACSAria+innards.jpg Images - WCHRI Aria II - Randall lost an O'ring! :P https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/59d60e03f9a61e6e954b3557/1507261343130/unnamed-1.jpg Images - First Cytometry "facility" in Adelaide https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/59d379af197aea90a304a0e6/5a69323c24a6946fda02baeb/1516843915161/IMG_6812.jpg Images - Inaugural CYTOMAD Gathering A wonderful group of cytometrists from far and wide in Adelaide joined us at our first event - more than 30 people from different cytometry interests came along to listen to Dr Stephen Blake and Ms Natalie Sanders. https://www.cytomad.com/about daily 0.75 2017-10-13 https://www.cytomad.com/meetings daily 0.75 2018-05-09 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59d60a0ac534a58fca7c0056/1507199534007/outline.png EVENTS https://www.cytomad.com/detmold-cytometry daily 0.75 2017-10-13 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59d602ee37c581acfceccebe/1507503400819/DFCF+logo+wavelength+no+background.png Detmold Family Cytometry Facility https://www.cytomad.com/sahmri-cytometry daily 0.75 2017-10-13 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59d60d108419c252d62bd1ae/1507201081426/SAHMRICytometry.png SAHMRI Cytometry https://www.cytomad.com/flinders-cytometry daily 0.75 2017-10-18 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59d610612994ca2bba7d5e0e/1507201143233/FU_V_RGB_L.png Flinders Cytometry https://www.cytomad.com/uofa-cytometry daily 0.75 2017-10-13 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59d611acbce1767a9d96b1f7/1507201578657/UoA_logo_col_vert.png UofA Cytometry https://www.cytomad.com/beckman-coulter daily 0.75 2018-05-09 https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/59d6f1c87131a5cab6f04887/1507260030603/BeckmanCoulter.png SPONSORS https://static1.squarespace.com/static/59d0bff1cd39c3d497ea3ca1/t/5af2f3b58a922dd314294149/1525871830259/Update_Color_BD_PNG_Logo.png SPONSORS